Effect of Activation and Inhibition of Cellular PKR on Coxsackievirus B3 Replication
نویسندگان
چکیده مقاله:
The ds-RNA activated protein kinase (PKR) is a serine-threonine kinase with MW of 68 KDa. It belongs to a family of kinases that control one of the translational initiation factors, eIF2. PKR is produced at high level in response to viral infection. This protein by phosphorylating eIF2 inhibits cellular protein synthesis. In this study, the effect of gamma interferon (IFN-γ), an activator, and 2-aminopurine (2AP), an inhibitor of PKR production on coxsackievirus B3 (CVB3) replication were studied. After addition of IFN-γ and 2AP to Vero and HeLa cultures, cells were infected with CVB3. After 48 h and appearance of cytopathic effect (CPE), cells were collected and viral replication was assessed by standard method. For molecular detection of PKR, RT-PCR technique was used. The data show that interferon inhibited (or lowered) and 2AP promoted viral replication, though in all samples presence of PKR-mRNA could be detected. It seems that PKR plays a key role in CVB3 replication. Therefore, PKR can be considered as a promising and considerable target for designing small molecules and drugs against CVB3
منابع مشابه
effect of activation and inhibition of cellular pkr on coxsackievirus b3 replication
the ds-rna activated protein kinase (pkr) is a serine-threonine kinase with mw of 68 kda. it belongs to a family of kinases that control one of the translational initiation factors, eif2. pkr is produced at high level in response to viral infection. this protein by phosphorylating eif2 inhibits cellular protein synthesis. in this study, the effect of gamma interferon (ifn-γ), an activator, and ...
متن کاملthe effect of explicit teaching of metacognitive vocabulary learning strategies on recall and retention of idioms
چکیده ندارد.
15 صفحه اولeffect of seed priming and irrigation regimes on yield,yield components and quality of safflowers cultivars
این مطالعه در سال 1386-87 در آزمایشگاه و مزرعه پژوهشی دانشگاه صنعتی اصفهان به منظور تعیین مناسب ترین تیمار بذری و ارزیابی اثر پرایمینگ بر روی سه رقم گلرنگ تحت سه رژیم آبیاری انجام گرفت. برخی از مطالعات اثرات سودمند پرایمینگ بذر را بر روی گیاهان مختلف بررسی کرده اند اما در حال حاضر اطلاعات کمی در مورد خصوصیات مربوط به جوانه زنی، مراحل نموی، عملکرد و خصوصیات کمی و کیفی بذور تیمار شده ژنوتیپ های م...
effect of postharvest application of chitosan and calcium chloride on decay and quality attributes of sweet cherry
چکیده ندارد.
15 صفحه اولeffect of oral presentation on development of l2 learners grammar
this experimental study has been conducted to test the effect of oral presentation on the development of l2 learners grammar. but this oral presentation is not merely a deductive instruction of grammatical points, in this presentation two hypotheses of krashen (input and low filter hypotheses), stevicks viewpoints on grammar explanation and correction and widdowsons opinion on limited use of l1...
15 صفحه اولمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ذخیره در منابع من قبلا به منابع من ذحیره شده{@ msg_add @}
عنوان ژورنال
دوره 8 شماره 3
صفحات 157- 160
تاریخ انتشار 2004-07
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023